2007•Journal of Jinan UniversityRequires access

Isolation,Identification and characteristics of crude oil-degrading microorganism

Yan Jia

Abstract

Five bacterial strains(P1,P2,P3,P4,P5)which could efficiently degrade crude oil were isolated from oil-containing wastewater.The results of configuration observation,physiological and biochemical characteristics showed that these five strains were identified as Arthrobacter sp.,Plesiomonas sp., Pseudomonas sp.,Bacillus sp.and Xanthomonas sp.,respectively. P1,P2,P3,P4 and P5 had a crude oil degradation capability of 50.20%,55.59%,61.90%,55.66% and 55.95% when the oil concentration of weight was 10 g/L, after incubating in a medium containing crude oil as carbon source for seven days under pH=9,7,8,8 and 8 respectively.The inoculation amount,salinity,oxygen,temperature,oil concentration of weight and nutritious salt have significant effect on oil degradation.GC-MS analysis of residual oil showed that C7~C17 straight chain alkanes were easily degraded by the strains.

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Key concepts: Arthrobacter, Pseudomonas, Food science, Microorganism, Salinity, Chemistry, Residual oil, Isolation (microbiology)