Determination of Four β_2-Agonists Residues in Sausage by Matrix Solid-phase Dispersion and High Performance Liquid Chromatography-Tandem Mass Spectrometry

A method based on matrix solid-phase dispersion(MSPD) and high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) using isotope dilution technique was developed for the simultaneous determination of four β2-agonists,e.g.clenbuterol,ractopamine,salbutamol and terbutaline in sausage sample.The sausage samples were graphitized with C18 and eluted with methanol.After enzymolysis,the extracts was purified with solid-phase extraction MCX cartridges.The separation of analytes was performed by HPLC,and the identification of target compounds was achieved under multiple-reaction monitoring mode with electrospray ionization in positive mode.Four β2-agonists were quantified by the internal standard method.The calibration curves showed good linearities for four β2-agonists in the range of 0.2-20.0 μg/L with correlation coefficients(r2) more than 0.990.The limits of detection were 0.10 μg/kg for salbutamol and clenbuterol,and 0.15 μg/kg for terbutaline and ractopamine.The spiked recoveries ranged from 80% to 109% with relative standard deviations less than 10%.The method showed the advantages of simplicity,sensitivity and less consumption of sample and organic solvents,and could meet the requirements for the determination of clenbuterol,ractopamine,salbutamol and terbutaline in sausage sample.