Relationships Study about Polymorphism of Matrix Metalloproteinase-9 with Coronary Heart Disease

Objective:To examine the matrix metalloproteinase-9 (MMP-9) C-1562T polymorphism in Shanxi province and study the relationships of this genetic variation with CHD.Method:Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for the detection of MMP-9 genotype on 64 CHD patients, including 37 myocardial infarction (MI) and 27 unstable angina (UA), and 84 healthy control subjects, and then the frequency of genotype was statistically computed.Result:Three genotypes of MMP-9, C/C、C/T、T/T had been detected in both groups. The frequencies of C/T genotype and T allele in CHD group were 26.6 % and 14.8 % respectively, while 21.4 %, 10.7 % in control group, there was no difference between them in statistics. When analyzed by subgroup, UA group (C/T 44.4 %, T 25.9 %) were significantly higher than those in the control group(C/T 21.4 %, T 10.7 %) ( P 0.05 ).Conclusion:The frequencies of C/T genotype and T allele of UA group in CHD were significantly higher than those in the control group, while in clinic, in the patients of C/T and T/T genotype which take one T allele at least, the proportion of below 65 years old was 61%, and among them, mostly were UA patients ( 72.7 %). This indicate that MMP-9 C-1562T may be an impressibility factor of UA below 65 years old in CHD.