The Effect of Astragalus Injection on the expression of HIF-1α and VEGF in the Cerebral Tissue after Focal Cerebral Ischemia

Objective To observe the effects of astragalus injection on the expression of HIF-1α and VEGF in the cerebral tissue after focal cerebral ischemia reperfusion in rats.Methods Thirty male SD rats with average body weight of 260-300g were randomly divided into three groups: sham-operation group,ischemic model group and astragalus injection group.The model of the middle cerebral artery occlusion in rats was performed with the suture occlusion for two hours.The expression of HIF-1α and VEGF were detected by using reverse transcription-polymerase chain reaction(RT-PCR).Results Compared with sham-operation group,the expression of HIF-1α and VEGF increased in the ischemic model group,and compared with ischemic model group,astragalus injection could significantly raise the expression of HIF-1α and VEGF(P0.01 and P0.05,respectively).Conclusions These results suggested that the astragalus injection ccould significantly protect cranial nerve in ischemic penumbra around ischemia reperfusion cerebral tissue in rats.It may through up-regulating the expression of HIF-1α and VEGF.