The protection research of Rubidate for the hypoxia /reoxygenation injury of the cardiomyocytes

Objective To investigate if Rubidate can protect myocardium cells which accompany with hypoxia/reoxygenation in neonatal rat myocardial. Methods Myocardium cells of neonatal rat were primary cultured, hypoxia and reoxygenation model were established, activity of lactate dehydrogenase (LDH), malondialdehyde (MDA), and rates of cell apoptosis and necrosis were detected respectively. Results Compared with hypoxia / reoxygenation model group, we found that the activity of LDH and MDA in hypoxia / reoxygenation-madder group were reduced more than hypoxia / reoxygenation group,the difference was statistically significant (P0.05). The rates of cell apoptosis and necrosis were less in hypoxia / reoxygenation-madder group than hypoxia / reoxygenation group. Conclusion Rubidate can protect myocardium cells which accompany with hypoxia/reoxygenation and inhibiting cell apoptosis in vitro.