Effects of Genistein on proliferation,differentiation,content of matrix calcium and mineralization of cultured osteoblasts in vitro

Objective To study the effects of Genisteina kind of substance from isoflavont on cultured osteoblasts in vitro. Methods MTT,PNPP,atomic absorptiometry and ARS were used to observe the proliferation,activity of ALP,contents of matrix calcium and the number of mineral nodes of osteoblasts cultured in vitro . Results It was found that Genistein had the effects on stimulating the proliferation,ALP activity,increasing the contents of matrix calcium and the number of mineral nodes of cultured osteoblasts. Conclusion Genistein has the effects on stimulating the proliferation,differentiation,maturation and mineralization of cultured osteoblasts in vitro. \;