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2010•Journal of Apoplexy and Nervous DiseasesRequires access

Significance of PI3K/Akt signal in apoptotic cells mimic Alzheimer's disease and the influence of edaravone

HE Dan-da

Open publisher page 2 citations

Abstract

Objective To investigate the effects of edaravone(MCI-186)on apoptosis of PC12 cells induced by β-amyloid(Aβ)and its possible relationship with PI3K/Akt.Methods The flow cytometry analysis was used to detect apoptosis,and Western blot was used to detect Akt phosphorylation level and total Akt for observing the protective effect of MCI-186 on Aβ induced apoptosis in PC12 cells.Results Akt Ser473 decreased in group of model at different time points compared to normal control group(P0.05),Akt Ser473 increased and apoptosis rate was decreased significantly in protection group(P0.01).Conclusion Aβ1-40 induced PC12 cells apoptosis through inhibiting Akt phosphorylation level;on the contrast,MCI-186 protectes against Aβ-induced cytotoxicity and decrease apoptosis cells under Aβ1-40 treatment by PI-3K/Akt signal transduction pathway.

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Objective To investigate the effects of edaravone(MCI-186)on apoptosis of PC12 cells induced by β-amyloid(Aβ)and its possible relationship with PI3K/Akt.Methods The flow cytometry analysis was used to detect apoptosis,and Western blot was used to detect Akt phosphorylation level and total Akt for observing the protective effect of MCI-186 on Aβ induced apoptosis in PC12 cells.Results Akt Ser473 decreased in group of model at different time points compared to normal control group(P0.05),Akt Ser473 increased and apoptosis rate was decreased significantly in protection group(P0.01).Conclusion Aβ1-40 induced PC12 cells apoptosis through inhibiting Akt phosphorylation level;on the contrast,MCI-186 protectes against Aβ-induced cytotoxicity and decrease apoptosis cells under Aβ1-40 treatment by PI-3K/Akt signal transduction pathway.

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Available abstract

Objective To investigate the effects of edaravone(MCI-186)on apoptosis of PC12 cells induced by β-amyloid(Aβ)and its possible relationship with PI3K/Akt.Methods The flow cytometry analysis was used to detect apoptosis,and Western blot was used to detect Akt phosphorylation level and total Akt for observing the protective effect of MCI-186 on Aβ induced apoptosis in PC12 cells.Results Akt Ser473 decreased in group of model at different time points compared to normal control group(P0.05),Akt Ser473 increased and apoptosis rate was decreased significantly in protection group(P0.01).Conclusion Aβ1-40 induced PC12 cells apoptosis through inhibiting Akt phosphorylation level;on the contrast,MCI-186 protectes against Aβ-induced cytotoxicity and decrease apoptosis cells under Aβ1-40 treatment by PI-3K/Akt signal transduction pathway.

Key concepts: Protein kinase B, Apoptosis, PI3K/AKT/mTOR pathway, Phosphorylation, Flow cytometry, Edaravone, Western blot, Chemistry

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