Determination of Phenobarbitone Concentrati in Plasma by RP-HPLC

Objective:To establish a method for rapid determinination of phenobarbitone in human plasma.Methods:RP HPLC with carbamazepine as intemal standard was used to determine the concentration of phenobaritone in human plasma.The separation was performed on a shimadzu shimpack CLC C 18 column with methanol water (60∶40) as mobile phase with a flow rate of 0.8 ml/min.The wavelength for measurement was set at 254 nm. Results:The linear range of phenobarbitone was between 5～40 mg/L with r= 0.999?9 .The lowest limit of detection was 11.57 μg/L.The average rates for phenobarbitone recovery in three different concentrations were 100.24%,100.28%,99.41%,with RSD of 0.7%,2.7% and 5.8%,respectively (n=9).The RSD values of intraday and interday were 2.4%,6.3%,8.6% and 5.7%,4.8%,7.2% (n=5). Conclusion:This method is accurate,rapid and easy and can be used as a roution method by TDM.