Optimization of Aqueous Enzymatic Extraction of Polysaccharides from Rapeseed Meal by Response Surface Methodology

The aqueous enzymatic extraction of polysaccharides from rapeseed meal was optimized by response surface methodology.One-factor-at-a-time method was employed to investigate the effects of five operating parameters on polysaccharide yield.The optimum ratio of water to material was 25:1(mL/g).Further,a quadratic polynomial regression model describing polysaccharide yield as a function of enzyme dosage,pH,temperature and extraction time was developed using the SAS software based on a Box-Behnken experimental design.The results of response surface analysis showed that the optimal extraction conditions were extraction at 62 ℃ and pH 6.7 for 79 min with an enzyme dosage of 256 U/g,resulting in a polysaccharide yield as high as 9.01%.