Determination of Imidaeloprid Pesticide Residue in Fresh Vegetables by HPLC

A novel high performance liquid chromatography(HPLC) method was developed for the analysis of imidaeloprid pesticide residue in fresh vegetables.Imidaeloprid was extracted from the samples by acetonitrile,purified with LC-NH2 column and determined by high performance liquid chromatography with UV detection.Symmetry Shield? C18 column(4.6×250 mm,5 μm) using the mixture of methanol,water and acetonitrile(55:25:20) as the mobile phase.The column temperature,flow rate,detection wavelength and sample volume were 30.0 ℃,0.8 mL/min,275 nm and 10.0 μL,respectively.Within the linear range of 0.1~1.0 mg/L,the correlation coefficient,the recoveries of imidaeloprid in tomato and the relative standard deviations(RSD) were 0.9925,95.4%~106.8 % and 2.66%~7.99%,respectively.The method was simple,effective and accurate.It can be applied to determinate imidaeloprid pesticide residue in fresh vegetables.