2011•Journal of Sichuan UniversityRequires access

The tolerant responses to NaCl Stress in IrrE-transgenic Brassica napus

Feng Bin

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Abstract

The tolerant responses to NaCl stress in IrrE-transgenic Brassica napus were studied.The results showed that the activity of peroxidas(POD),superoxide dismutase(SOD) and catalase(CAT) in transgenic Brassica napus was gradually increased under 200 mmol/L NaCl during 0~48 h,but the activity of POD,SOD and CAT in non-transgenic Brassica napus was increased during 0~24 h and subsequently decreased after 24 h.The content of proline and the dissoluble proein in transgenic Brassica napus was higher than non-transgenic Brassica napus,but the content of malonaldehyde(MDA) in transgenic Brassica napus was lower than non-transgenic Brassica napus.Therefore,IrrE gene enhanced the activities of three antioxidant enzymes in transgenic Brassica napus,and then enhanced the tolerance of Brassica napus against NaCl stress.

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What this paper is about

The tolerant responses to NaCl stress in IrrE-transgenic Brassica napus were studied.The results showed that the activity of peroxidas(POD),superoxide dismutase(SOD) and catalase(CAT) in transgenic Brassica napus was gradually increased under 200 mmol/L NaCl during 0~48 h,but the activity of POD,SOD and CAT in non-transgenic Brassica napus was increased during 0~24 h and subsequently decreased after 24 h.The content of proline and the dissoluble proein in transgenic Brassica napus was higher than non-transgenic Brassica napus,but the content of malonaldehyde(MDA) in transgenic Brassica napus was lower than non-transgenic Brassica napus.Therefore,IrrE gene enhanced the activities of three antioxidant enzymes in transgenic Brassica napus,and then enhanced the tolerance of Brassica napus against NaCl stress.

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Available abstract

The tolerant responses to NaCl stress in IrrE-transgenic Brassica napus were studied.The results showed that the activity of peroxidas(POD),superoxide dismutase(SOD) and catalase(CAT) in transgenic Brassica napus was gradually increased under 200 mmol/L NaCl during 0~48 h,but the activity of POD,SOD and CAT in non-transgenic Brassica napus was increased during 0~24 h and subsequently decreased after 24 h.The content of proline and the dissoluble proein in transgenic Brassica napus was higher than non-transgenic Brassica napus,but the content of malonaldehyde(MDA) in transgenic Brassica napus was lower than non-transgenic Brassica napus.Therefore,IrrE gene enhanced the activities of three antioxidant enzymes in transgenic Brassica napus,and then enhanced the tolerance of Brassica napus against NaCl stress.

Key concepts: Brassica, Transgene, Superoxide dismutase, Canola, Point of delivery, Genetically modified crops, Biology, Catalase

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