Effect of TSA on the proliferation,apoptosis and expression of FHIT in human hepatoma cell line HepG2

Objective To observe the effects of histone deacetylase inhibitor trichostatin A(TSA) on the growth of human hepatoma cell line HepG2 and its mechanism.Methods Human hepatoma HepG2 cells cultured in logarithmic phase were divided randomly into TSA group,control group and blank group,the TSA group was treated with TSA at the concentrations of 125,250,500,1 000,2 000 nmol/L,the control group was treated with equal volume of DMSO,and the blank group was treated with only medium without cells,24 to 72 hours later,the cell proliferation inhibition rate(IR) was analyzed by MTT assay,the cell morphology was observed by the inverted light microscope and electron-microscopy,and the apoptosis index was determined by using TUNEL technique.The expression of FHIT gene and protein was quantified using real-time PCR and Western blot.Results Compared with the control group,IR of the TSA group increased in dose and time-depended manner(P0.05),early apoptosis of the HepG2 cells was observed under electron microscope;compared with the control group,the apoptosis rate of the TSA group increased,the expression of FHIT mRNA and protein increased(P0.01).Conclusion TSA can inhibit the proliferation and promote apoptosis of hepatocarcinoma cell HepG2,the mechanism may relate to the up-regulating of FHIT expression.