Study on commonly used rat model of blood stasis syndrome

Objective To compare the hemorheology indexes and coagulation parameters of 6 rat models of blood stasis syndrome which were established by using six different methods,and to discuss the characteristics and applicability of each rat model. Methods Six different rat models of blood stasis syndrome were established including congealing cold with blood stasis group, adrenaline group, dextranum group,hyperlipidemia with congealing cold group,hyperlipidemia group and blood deficiency with blood stasis group. The differences in whole blood viscosity( WBV),plasma viscosity,erythrocyte deformability,hematocrit( HCT), platelet aggregation,prothrombin time( PT), activated partial thromboplastin time( APTT) and fibrinogen( FIB) content were compared among all groups. Results The hemorheology indexes and coagulation parameters were improved in adrenaline group,hyperlipidemia group,hyperlipidemia group and blood deficiency with blood stasis group,which was more significant in adrenaline group with higher applicability. The hemorheology indexes were significantly influenced in dextranum group,especially RBC-related parameters. The coagulation parameters were significantly influenced in congealing cold with blood stasis group. Conclusion The rat models of blood stasis syndrome established by using different methods show different characteristics in hemorheology indexes and coagulation parameters,and have different applicability. This study provides some reference for choosing rat models of blood stasis syndrome in the research on the activities of medicinals with the actions of activating blood and resolving stasis.