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2015•Journal of Hubei University of Chinese MedicineRequires access

Determintion of Isofraxidin in Gubisan by RP-HPLC

WU Yaohu

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Abstract

Objective To analyze the content of Isofraxidin in Gubisan. Methods The DIONEX- C18column( 250 mm × 4. 6mm,5μm) was used. The mobile phase consisted of acetonitrile- 0. 1% phosphoric acid water( 25: 75). The flow rate was 1. 0m L / min. The column temperature was 30℃. The UV detector wavelength was 300 nm. Results The linear ranges of Isofraxidin was 10. 4208- 74. 1385μg( r= 0. 9997) with average recovery of 99. 1143%( RSD = 0. 6623%). Conclusions The method is stable,simple and simple,which could be used for the quality control and determination of content of Gubisan.

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Objective To analyze the content of Isofraxidin in Gubisan. Methods The DIONEX- C18column( 250 mm × 4. 6mm,5μm) was used. The mobile phase consisted of acetonitrile- 0. 1% phosphoric acid water( 25: 75). The flow rate was 1. 0m L / min. The column temperature was 30℃. The UV detector wavelength was 300 nm. Results The linear ranges of Isofraxidin was 10. 4208- 74. 1385μg( r= 0. 9997) with average recovery of 99. 1143%( RSD = 0. 6623%). Conclusions The method is stable,simple and simple,which could be used for the quality control and determination of content of Gubisan.

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Available abstract

Objective To analyze the content of Isofraxidin in Gubisan. Methods The DIONEX- C18column( 250 mm × 4. 6mm,5μm) was used. The mobile phase consisted of acetonitrile- 0. 1% phosphoric acid water( 25: 75). The flow rate was 1. 0m L / min. The column temperature was 30℃. The UV detector wavelength was 300 nm. Results The linear ranges of Isofraxidin was 10. 4208- 74. 1385μg( r= 0. 9997) with average recovery of 99. 1143%( RSD = 0. 6623%). Conclusions The method is stable,simple and simple,which could be used for the quality control and determination of content of Gubisan.

Key concepts: Phosphoric acid, Chromatography, High-performance liquid chromatography, Chemistry, Acetonitrile, Content determination, Analytical Chemistry (journal), Organic chemistry

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