Screening and Identification of Functional Gene Sets in Aged Kidney-Yang Deficiency Syndrome:cDNA microarray assay

Objective:To screen and identify functional gene sets in aged kidney-Yang deficiency syndrome by cDNA microarray assay.Methods:Two aged kidney-Yang deficiency patients and two healthy peoples were selected as samples during the epidemiologic survey of the kidney-Yang deficiency syndrome.The differentially expressed genes were screened by applying HG-u133Plus2.0 of Affymetrix GeneChip.The total RNAs were isolated from leukocyte of 4 samples by TR Izolmethod and then purified,reversely transcribed to cDNA with incorporating biotin labeling probe and hybridizated with GeneChip.Picture signals of fluorescence in gene array were scanned and compared differential expression of genes by computer analysis.Results:680 and 503 differentially expressed genes were obtained.The immune response-associated gene sets were found with significance in GO annotated biological processes.Conclusion:Immune factor play an important role in occurrence and development of the kidney-Yang deficiency.