Preparation of Collagen Peptide from Pig Skin Hydrolyzed by Papain

The process of preparing of collagen peptide from pig skin by papain was studied.The factors of affecting process of hydrolysis were investigated.By determining the hydrolysis degree of collagen protein(the ratio of the peptide bonds eleavaged /total peptide bonds in protein),it could be concluded that the best conditions for the hydrolysis of pig skin were:pH 5,hydrolyzing temperature 60 ℃,ratio of papain 0.9 %,concentration of substrate 30% and total hydrolysis time 4 h.Under these conditions the hydrolysis degree of collagen protein could reach 14.91%.