Effect of EGCG on apoptosis of cervical cancer Hela cells induced by DDPand the related mechanism of tumor cell apoptosis

Objective:To study the different effects of EGCG alone or its cooperation with DDP on in vitro growth of Hela cells of human cervical carcinoma.Methods:In this experiment, MTT colorimetry was adopted to examine the inhibitory effect of drugs on cell growth,and the morphological changes of cell apoptosis were observed by HE method.The rate of cell apoptosis was detected with TUNEL method.The expression of albumen NF-κB and Cyclins D1 was investigated by immunohistochemical techniques.Results:The inhibitory ratio was 12.6%, after 50μg/mL of EGCG was added to in vitro growing Hela cell lines for 12 h.The inhibitory ratio was 78.3%,after 400μg/mL of EGCG was added to in vitro growing Hela cell lines for 48h(P0.05).The apoptosis inhibition ratio was 13.7%(P0.05) when the 50μg/mL of EGCG was jointly used with 0.1μg/mL DDP for 12h.The effect was stronger than when EGCG alone or DDP alone was used(P0.05).EGCG could decrease the expression of NF-kB and Cyclins D1(P0.05).Conclusion:EGCG can inhibit the Hela cell growth and induce the cell apoptosis in human cervical carcinoma. EGCG has an enhanced sensitivity effect on DDP,and EGCG could decrease the expression of NF-kB and Cyclins D1.