EGCG Sensitisizes Cisplatin-resistant Human Lung Adenocarcinoma Cells A549/DDP to Cisplatin

Objective To investigate the effect of EGCG on apoptosis and drug sensitization in cisplatin-resistant human lung adenocarcinoma cell line A549/DDP and to determine the inhibitory effect of EGCG in cells A549/DDP. Methods Cytotoxicity of EGCG and sensibility to doxo-rubicin(DOX) in A549/DDP cell line,were studied by MTT assay.AO/EB method was used to display the apoptosis morphological of A549/DDP cell induces by EGCG.The effect of EGCG on inducing of apoptosis was analyzed by flow cytometry(FCM).Western blot analysis was performed to detect the expresssion of GST-π,Survivin protein after EGCG treatment. Results After 24 hours exposure to EGCG,partial cells presented the characteristic morphological changes of apoptosis under the light microscopy including cell shrinkage,nuclear condensation.Western Blot analysis showed that GST-π and Survivin were decreased,after to EGCG(20,40,60 μg/ml)Analysis by flow cytometry showed that the percentages of apoptosis cells were increased. Conclusion EGCG can sensitize cells to DDP increase cell spontaneous,which might be relate to the performed to detect the expresssion of GST-π、Survivin,and could significantly induce apotosis by this regulation