2012•Zhongguo renshougonghuanbing zazhiRequires access
Pathogenic characterization of Shigella isolated from Ningxia
Liang Junrong
Open publisher page 0 citations
Abstract
The objective of the present study was to investigate the distribution and drug susceptibility of Shigella bacteria in Ningxia,and provide the basis for prevention and control of bacillary dysentery through homology analysis of molecular epidemiology.Biochemistry of API20E system was used to identify strains,and the bacteria categories were tested by slide agglutination with a serum.The sensitivity of strain to 10 commonly used antibiotics was detected with Kirby-Bauer assay,and pulsed-field gel electrophoresis(PFGE) was used for molecular typing.The results were analysed by BioNumerics V4.0 and UPGMA method.Among the 176 Shigella,there were 64 Shigella sonnet(36.36%) and 112 Shigella flexneri(63.64%) which containing 52 Shigella flexneri 2b(29.55%).Results showed that drugs with higher resistance rate were ampicillin,nalidixic acid,tetracycline and rifampin,following by amoxicillin and cotrimoxazole.The most sensitive drugs were ciprofloxacin(CIP),cefotaxime and cephalothin;the test also showed that Shigella flexneri and Shigella sonnet had slight difference in resistance spectrum to 10 antibiotics.The 112 Shigella flexneri were divided into 36 banding patterns,in which JZXN11.NX06,JZXN11.NX07,JZXN11.NX021,JZXN11 and NX0025,accounting for 57.14%,were the main outbreak types in Ningxia.The 64 Shigella sonnet were divided into 21 banding patterns,in which J16X01.NX0011 and J16X01.NX0012 were the 2 epidemic banding patterns,accounting for 31.25% and 15.63% respectively.It indicates that in Ningxia,the most popular Shigella bacteria is Shigella flexneri,and F2b is the dominant serotype.The detection rate of F4c and Shigella sonnet have decreased significantly.The drug resistance rate of Shigella to ampicillin(AMP),tetracycline(Te) and amoxicillin(AML) are the highest,and the situation of multi-drug resistance is serious.The PFGE have type presented a diverse epidemic pattern.The advantaged bands are less popular than before and there is coexistence for various types.