Simplified method for determination of hexaflumuron residues in cotton and soil using QuEChERS and UPLC-MS/MS

A rapid method for the determination of hexaflumuron residues in cotton and soil was established based on matrix solid-phase dispersion(QuEChERS) and ultraperformance liquid chromatography-mass spectrometry(UPLC-MS/MS).The sample was extracted by acetonitrile and purified on primary secondary amine(PSA).The mass spectrometer was operated with electrospray in the opposite ion mode(ESI-).The target analytes were quantified by external standard method and matrix-matched standard solution used to compensate for matrix effect.Average recoveries of hexaflumuron in soil and corn samples were found in the range of 71.1%-110.0% at three spiking levels from 0.005 mg/kg to 0.5 mg/kg with relative standard deviations of 2.7%-8.4%.Limits of detections(LOD) of hexaflumuron on soil,cotton leaf and cotton seed were 0.04,0.12 and 0.22 μg/kg,while limits of quantifications(LOQ) were 0.14,0.41 and 0.74 μg/kg.The method is simple and suitable for the routine and confirmation analysis on cotton leaf,cotton seed and soil.