Studies on Thermostable and Acid Lipase Produced by Pichia anomala

lipase-producing strains were selected from 1 1 3 7yeastthrough Rhodamine B dye solid plate assay method.A high lipase-yielding strain of 1 0 1 3 was obtained by liquid reselection from 2 4strains with largertransparentcycles.In addition,the optimal lipase producing conditionsof strain 1 0 1 3 were as following:Soy bean meal2 .0 % ,wheatbran1 .0 % ,NH4 NO30 .4% ,KH2 PO4 0 .3 % , Mg SO4 · 7H2 O 0 .2 5 % ,soybean oil 1 .0 % . The optimal conditions for lipase were initial p H 5 .8, culture 2 8℃ for 3 6h. The lipase activity for hydrolysis of olive is optimal at p H 4.8and at 48℃ , respectively,the lipase was stable below 5 0℃ for 40 min at p H 4.8. Different metal ions showed adverse effects on the lipase activity.Mg2 + and Na+ enhanced the lipase activity,whereas Zn2 + 、Fe2 + 、 Cu2 + and EDTA cause inhibition.