[Conditions of enzyme production and properties of alkaline lipase by Streptomyces Z94-2].

An alkaline lipase producing strain streptomyces Z94-2 was selected from 152 lipase producer, The optimal medium composition for the producing lipase is (g/L): soybean meal 30, urea 10, dextrin 10, KH2PO4 0.5, NaCl 1, MgSO4.7H2O 0.5, AEO9 (nonionic surfactant) 1. The optimal conditions for lipase producing are initial pH 9.5-10.0, shaken at 26 degrees C, for 48 h, a maximal lipase activity of reached 596 (u/mL). The optimal activity at pH 9.8 and at 37 degrees C, respectively. The lipase was stable at pH 8.6-10.2 and below 60 degrees C, and the lipase was highly activated by 0.14 mol/L CaCl2.