Simultaneous Analysis of Isoprothiolane and Tricyclazole in Rice and Soil by High Performance Liquid Chromatography

The quantitative analysis method of isoprothiolane and tricyclazole was developed using high performance liquid chromatography(HPLC) with ultraviolet absorbance detector and a SunFireTMC18 column.Samples were extracted under ultrasonic with methanol and acetone,purified on the Florisil column.The mobile phase was methanol-water(80∶20,V/V) at a flow rate of 0.5 mL/min.The wavelength of detector was set at 260 nm.The results showed that these compounds could be effectively isolated.The linear correlation coefficient for isoprothiolane and tricyclazole was 0.999 8 and 0.999 9,respectively.The detection limit of isoprothiolane and tricyclazole was 1.6 and 1.3 ng,respectively.The recoveries of isoprothiolane and tricyclazole in rice were 84.78%～93.35% and 86.75%～91.13%,respectively,and relative standard deviations(RSDs) were 2.15%～5.81% and 2.46%～4.94%,respectively.The recoveries in soil were 89.65%～94.08% and 88.81%～91.06%,respectively;and RSDs were 2.19%～4.31% and 3.08%～5.38%,respectively.The method can be used for conventional analysis,which has rapid,simple,accurate and sensitive characteristics.