2001•Journal of Modern RehabilitationRequires access
Investigation of the law and phenomena about apoptosis of osteoclasts of SD rat cultured in vitro
De Chen
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Abstract
Objective To study the phenomena and the law of apoptosis about osteoclasts of SD rat cultured in vitro. Method The phenomena about apoptosis of osteoclasts of SD rat cultured in vitro were investigated through morpholog and DNA 3－OH terminal deoxynucleotidyl transferase－mediated deoxyuride tiphosphate nick－end labeling by microscope, and the law of apoptosis was suspected. Result The phenomena of apoptosis was observed, the number and the ratio of osteoclasts in apoptosis were increasing by the time of culture. The ratio of apoptosis of osteoclasts were 10, 14, 34 and 50 percent at the date of 3, 4, 5 and 6 respectively. Conclusion Apoptosis is existed in osteoclasts of SD rat cultured in vitro, the ratio of apoptosis of osteoclasts is increasing by the time of culture.