Influence of adiponectin on mRNA levels of adhesion factors in umbilical vein endothelial cells

Objective To investigate the effect of adiponectin on the mRNA level of adhesion factor in human umbilical vein endothelial cells(HUVECs) induced by TNF-α.Methods HUVECs were cultured in vitro and divided into normal group,TNF-α stimulation group and adiponectin and TNF-α associated stimulation group.For adiponectin and TNF-α associated stimulation group,HUVECs were preincubated with human recombinant adiponectin(5,30 or 50 mg/L) for 16 h,then exposed to human recombinant TNF-α(20 μg/L) for another 6 h,which was then compared with TNF-α stimulation groups(10,20 or 50 μg/L) in the mRNA levels of vascular adhesion molecule-1(VCAM-1),intracellular adhesion molecule-1(ICAM-1) and E-selectin by PCR.Results TNF-α stimulation group had increased mRNA levels of VCAM-1,ICAM-1 and E-selectin.Compared with TNF-α stimulation group,the mRNA levels of VCAM-1,ICAM-1 and E-selectin in adiponectin and TNF-α associated stimulation group were decreased.Conclusion Adiponectin attenuates the mRNA levels of adhesion factors,such as VCAM-1,ICAM-1 and E-selectin,in umbilical vein endothelial cells induced by TNF-α,and adiponectin has protective actions against the initiation and progression of atherosclerosis.