Effect of atorvastatin on apoptosis of hyperglycemia induced mesangial cells in rat

Objective To investigate the influence of different concentrations of atorvastatin on apoptosis of rat mesangial cells.Methods Rat immortal mesangial cells were cultured in vitro and divided into 5groups:control(C)group,hyperglycemia(30 mmol/L,H)group,hyperglycemia(30 mmol/L)+atorvastatin(10-7 mmol/L)(H+A1)group,hyperglycemia(30 mmol/L)+atorvastatin(10-6 mmol/L)(H + A2)group and hyperglycemia(30mmol/L)+atorvastatin(10-5 mmol/L)(H+A3)group.Apoptosis was detected by Annexin V-FITC and PI double staining,and the caspase-3activity was assayed by the caspase-3activity kit.Results Compared with the group C,the mesangial cells apoptosis rate and caspase-3activity in the group H were significantly increased,the differences were statistically significant(P0.05).Compared with the group H,the mesangial cells apoptosis rate and caspase-3activity in the H+A1,2,3groups were significantly decreased,the differences were statistically significant(P0.05),moreover which showing the concentration dependence.Conclusion Atorvastatin may inhibit aldosterone induced mesangial cells apoptosis via caspase pathway.