EFFECTS OF DECORIN ON AIRWAY REMODELING IN ASTHMATIC MICE

Objective To study the effects of decorin on airway remodeling in asthmatic mice,and assess the value of decorin in the therapy of asthma.Methods Thirty female Kunming mice were evenly randomized to three grous as group A(control group),group B(asthma airway remodeling group) and group C(decorin intervention group).The mice in groups B and C were sensitized and repeatedly challenged with ovalbumin(OVA),those in group C were intervened with decorin.Transforming growth factor-β1(TGF-β1) in serum and bronchoalveolar lavage fluid(BALF) were measured by Enzyme-linked immunosorbent assay(ELISA).The pathological changes of the lung tissues were observed by haematoxylin-eosin(HE) staining.Collagen depositions were observed by Masson Trichrome staining.Indexes of airway remodeling were measured using image analysis system.The expressions of TGF-β1 in lung were detected by immunohistochemistry.Results TGF-β1 levels in serum and BALF,the thickness of total airway wall,inner airway wall,airway smooth muscle,and airway collagen deposition as well as TGF-β1 expression in lung tissue of mice in group B were significantly higher than that in group A(F=32.36-785.57,t=-18.01——7.86,P0.01),and that in group C were obviously lower than that in group B(t=5.58-9.57,P0.01).Conclusion Decorin can relieve airway remodeling in asthmatic mice.