Transforming growth factor-β_1 in serum,bronchoalveolar lavage fluid and lung tissue and airway remodeling in asthmatic rats

Objective To detect the different levels of transforming growth factor-β_1(TGF-β_1) in serum,bronchoalveolar lavage fluid(BALF) and lung tissue expression and its relationship with airway remodeling in asthmatic rats for the diagnosis and treatment of asthma and provide a theoretical basis.Methods Wistar rats were randomly divided into 2 groups:control group with 20 samples and the asthma model group 20 samples.Egg protein allergens were made into asthma and stimulation of rats.Elisa was used to measure the levels of TGF-β_1 in serum and BALF.Immunohistochemical was used to determine TGF-β_1 expression of the lung tissue and masson was used to observe collagen deposition.The computer image analysis system evaluated respiratory bronchiole smooth muscle thickness and the extent of epithelial damage score and other indicators of airway remodeling.Results After remodeling serum TGF-β_1 levels reduced significantly compared with those in control group,the result was significant(P0.05);BALF TGF-β_1 increased statistically(P0.01).TGF-β_1 of lung tissue expressions increased statistically (P0.01).Pathology examination of asthma rat airway epithelial damage,the thickness of airway smooth muscle,the airway deposition of collagen showed that airway remodeling of asthma model obviously increased than control group rat.Conclusion The increase levels of TGF-β_1 in BALF and lung tissue expression increases airway remodeling.The reduction levels in serum indicate that it is possible to detect the levels of TGF-β_1 in serum and may be useful in the diagnosis asthma and to understand airway remodeling.