STUDIES ON CELLULASE PRODUCTION BY BACILLUS SP.ZU- 04

Cellulase production was studied using a bacterial strain( Bacillus sp.ZU 04)screened at our laboratory.Cellulase activity could be detected from pH value 6.5 to 10.5.Corn corb residue from xylose industry was the suitable carbon source,and bean cake was the suitable nitrogen source for the strain.NaCl and KH 2 PO 4 were important to cellulase production,and their optimal mass parts were 0.5%-1.0% and 0.1%,respectively.Cellulase activity could be improved by addition of wheat bran.Optimal culture conditions in a 3 L fermenter were: stirring speed 300 r/min,aeration 0.3 L/(L·min),and culture temperature 37 ℃.Neutral and alkali cellulase activities detected from the fermentation broth were 257.6 U/mL and 125.6 U/mL,respectively.The enzyme had a good application potential.