Microsurgical technique of the model of orthotopic kidney transplantation in rat

Objective To study the microsurgical technique of establishing a reliable rat model of orthotopic kidney transplantation in rat.Methods The donors of the kidney were flushed with a total of 5 ml of 4 ℃ sodium lactate Ringer's injection through abdominal aorta.During orthotopic kidney transplantation,donor's renal artery,renal vein and ureter were joined to renal artery,vein and ureter of recipients with end-to-end anastomosis respectively.Results Rat orthotopic kidney transplantation were performed in 120 cases,and the successful rate of the model was 91.5%.In non-intervention group,the survival rate was satisfactory,with an 82.9% of 1-month survival.Conclusion The sophisticated microsurgical technique and the delicate surgical manipulation is the prerequisite of establishing orthotopic kidney transplantation model in rat.The model could be repeated stably and be suitable for investigation of the immunity mechanism of organ transplantation.