Anti-TMV Active Substances from Coreopsis drummondii

Production of plant secondary metabolites as a rich source of antimicrobial agents has been extensively applied in Chinese traditional medicine to treat viral diseases.Therefore,direct selection of antiphytoviral compounds from plants can be used to identify new potent antiviral agents.To screen antiviral compounds,tobacco mosaic virus(TMV) was chosen as a model target and the extracts with anti-TMV activity from 108 species of plants were analyzed.Among them,a extract from Coreopsis drummondii,predominantly suppressing the infection and replication of TMV was selected,and a constituent with antivirus activity was isolated from it by bioassay.The compound was identified as 1-phenyl-1,3,5-triheptalkyne using MS and NMR.Its activity against TMV was investigated by local lesion and leaf discs assay.The results indicated that the compound showed a signifi cant inhibitory activity against TMV in vitro,with 73.5% inhibitory rate against the infection of TMV and 84.3% against replication of TMV at a concentration of 0.2 mg/mL,while its inhibitory effect on TMV-CP gene expression in tobacco leaf discs was examined by real-time quantitative PCR,which showed that the compound obviously suppressed the expression of TMV-CP gene,with 79.8% inhibitory rate at the concentration of 0.2 ㎎/mL.Taken together,the anti-TMV activity of the compound contributes itself as a new potential candidate for development of antiviral drugs.Fig 6,Tab 1,Ref 19