Effect of Danlong Dingchuan Decoction on Expressions of MMP-9,TIMP-1 and Airway Remodeling in Asthmatic Mice

Objective:To Study the effect of Danlong Dingchuan decoction(DDD) on expressions of matrix metalloproteinases-9(MMP-9),tissue inhibitor-1 of metalloproteinase(TIMP-1) and airway remodeling in asthmatic mice.Method:Fifty six SPF BALB/c mice were randomly divided into normal group,model group,western medicine group and DDD group.The three groups except normal group were induced as asthmatic model in allergized mice with ovalbumin(OVA).During the 41-69 days in the experiment,the normal and model group were treated with normal saline.Meanwhile,the western medicine group and DDD group were respectivelytreated with dexamethasone and DDD.At the end of experiment,we observed the pathomorphological changes in lung issues and took the sample blood to detect MMP-9 and TIMP-1 using ELISA.Result: The pathological changes in lung tissue showed that DDD can significantly reduce the infiltration of inflammatory cells and inhibit bronchial smooth muscle thicken.The level of MMP-9 were significantly increased except the normal group(P0.05),and TIMP-1 in lung tissue were increased in model group(P0.01).In western medicine group and DDD group,the content of MMP-9 and TIMP-1 were significantly decreased(P0.01),but the relative value of MMP-9 and TIMP-1 in the two groups were significantly increased(P0.05).Conclusion: DDD can inhibit the secretion of MMP-9 and TIMP-1 which suppress airway remodeling in asthmatic mice.