Effect of 5-Aza-2'-deoxycytidine on human renal cell carcinoma cell line OS-RC-2

Objective To study the influence of 5-Aza-2'-deoxycytidine(5-Aza-CdR)on the proliferation and apoptosis of human renal carcinoma OS-RC-2 cell line.Methods Human renal carcinoma OS-RC-2 cells were treated with a cocentration of 10-7,10-6,10-5 or 10-4mol/L 5-Aza-CdR,respectively.The growth rate of the cells was detected by MTT assay and ultrastructural changes were observed under transmissional electron microscope.The apoptosis was analyzed by flow cytometry. Results 5-Aza-CdR inhibited the proliferation of OS-RC-2 cells in a time-and concentration-dependent manner(P0.05).There were significant increases in apoptotic rates of OS-RC-2 cells [(3.74±0.34)%,(7.85±0.59)%,(12.93±1.32)% vs.(0.86±0.08)%](P0.01) after 5-Aza-CdR treatment.The cells number of G0/G1 phase increased at a concentration of 10-6 mol/L(P0.05),and the cell cycle was stopped at G0/G1 phase after treated for 3 days. The methylation status of γ-catenin gene promoter region was reversed with 5-Aza-CdR 10-5mol/L treating for 72h.Conclusion 5-Aza-CdR can inhibit the growth of OS-RC-2 cells and promote their apoptosis by elimilating demethylation of γ-catenin gene CpG-rich promoter regions.