Effects of 5-Aza-CdR on proliferation of human renal carcinoma line OS-RC-2 and expression of γ-catenin protein

Objective: To investigate the effect of methylation inhibitor 5-Aza-2-deoxycytidine on the growth of human renal cancer cell line OS-RC-2 and γ-catenin expression.Methods: Human renal carcinoma OS-RC-2 cells were treated with different concentrations of 5-Aza-CdR(10-7,10-6,10-5,10-4 mol/L) respectively.Then the growth rate of the cells was detected by MTT assay.The apoptosis was analyzed by flow cytometry.The immuneocytochemistry was used to detect γ-catenin levels in the cell line before and after treatment with 5-Aza-CdR. Results: 5-Aza-CdR can inhibit the growth of OS-RC-2 cells,and the apoptotic rate was also increased after 5-Aza-CdR treatment.γ-catenin expression levels in OS-RC-2 cells were increased after 5-Aza-CdR treatment. Conclusion: γ-catenin expression levels were inhibited by methylation,5-Aza-CdR can inhibit the growth,and promote the apoptosis of OS-RC-2 cells by inducing demethylation in the promoter region of γ-catenin gene.