2013•Zhongguo linchuang yaolixue yu zhiliaoxueRequires access
Effects of naloxone on the expressions of JNK3 protein in rats after ischemia-reperfusion
Shen Jian-guo
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Abstract
AIM:To investigate the effect of naloxone on the expression of JNK3 protein in Hippocampus during transient focal cerebral ischemia reperfusion,and to discuss the probable mechanism of its protective effect.METHODS:The model of focal cerebral ischemia-reperfusion injury was established in rats by suture-occluded method.Rats were randomly divided into several groups,including Sham-operation,ischemia-reperfusion,low dose naloxone treated ischemia-reperfusion,high dose naloxone treated schemia-reperfusion,SP600125 treated ischemia-reperfusion and respective Vehicle treated ischemia-reperfusion.The infarction volume was detected by TTC staining.Western Blot was operated to detect expression of p-JNK3 in Hippocampus.RESULTS:Both groups of high dose naloxone treated ischemia-reperfusion and SP600125 treated ischemia-reperfusion,not only ischemia-reperfusion infarction volume was reduced,but also expression of p-JNK3 was down-regulated,compared with ischemia-reperfusion only group.As the dose increase,the effect of naloxone on inhibiting expression of p-JNK3 was obviously improved.CONCLUSION:Naloxone inhibits the expression of p-JNK3 in a dose-dependent manner during focal ischemia reperfusion which may be one of the mechanisms of its neuroprotective function.