Overexpression of tissue factor levels in plasma and tissue of hepato-cellular carcinoma(HCC)patients and its clinical significance

AIM:To detect tissue factor(TF)level both in plasma and in tissue of hepatocellular carcinoma(HCC)patients and to elucidate their association with clinical features.METHODS:Plasma TF levels of 50 cases of HCC patients and 30 cases of control were detected by ELISA.27 HCC tissue samples with their adjacent tissue samples and 27 normal liver tissues were detected by RT-PCR.RESULTS:① Plasma TF levels were increased significantly in HCC group when compared with control(P0.05).TF levels were higher in poor differentiation,large size and cirrhosis subgroup in HCC patients(P0.05).Plasma TF levels were also significantly increased in extra-hepatic metastasis,lymphatic metastasis and portal venous tumor thrombus subgroups(P0.05).② The mRNA expression rate of TF in HCC tissue was 62.96%(17/27)and the relative mRNA expression intensity of TF was 0.567±0.268.Those were significantly higher than that in their adjacent tissue samples or 27 normal liver tissue samples(P0.05).While the relative expressive intension of TF were also significantly higher in larger size and several invasive and metastatic subgroups(P0.05).CONCLUSION:TF might play an important role in hepatic carcinogenesis,invasiveness and metastasis.