EVALUATION ON GERMICIDAL EFFICACY OF ELECTROLYZED OXIDIZING WATER

Objective To study the germicidal efficacy of electrolyzed oxidizing water.Methods Suspension quantitative germicidal test and confocal laser scanning microscope(CLSM) were used to observe germicidal efficacy of electrolyzed oxidizing water.Results The electrolyzed oxidizing water could kill 100% of Bacillus subtilis var.niger spores in suspension with 15 min contact time under the clean conditions and only killed 99.65% after contact for 60 min with organic substance.Under the clean conditions,the killing rate of Staphylococcus aureus,Escherichia coli were all 100% after contact for 30 s.But when the 30 g/L calf serum protein was added in the suspension,the killing rate was 95.06% and 99.90% after contact for 12 min respectively.The germicidal efficacy of electrolyzed oxidizing water by means of CLSM observation was conformed with by means of suspension quantitative germicidal test.Conclusion The electrolyzed oxidizing water has good germicidal efficacy,and the effect is influenced obviously by organic substance.The germicidal efficacy result of electrolyzed oxidizing water by means of CLSM observation and suspension quantitative germicidal test are the same.