ScholarLens Back to search
2006•China Medical EngineeringRequires access

A modified alkaline lysis method for extraction of plasmid DNA

Liu Yong-he

Open publisher page 0 citations

Abstract

[Objective] Introduce a method for rapid extraction of Superior-Quality Plasmid DNA. [Method] Use alternative reagent to establish modified alkaline lysis method for extraction of plasmid DNA and use agarose electrophoresis to examine the quality of plasmid DNA, ultraviolet spectrophotometry to evaluate its production and purity, restricted enzyme cutting to examine its availability. [Results] The agarose electrophoresis showed that the plasmid DNA band was bright, mainly by form of superhelix. A260/280 was 1.903, DNA concentration was 320μg/ml, and we got 500 bp target fragment by restricted enzyme cutting. [Conclusion] We can get high purity of sample DNA by this simple and practical new method , it can meet the requirement of routine molecular biochemistry experiments.

About this research paper

What this paper is about

[Objective] Introduce a method for rapid extraction of Superior-Quality Plasmid DNA. [Method] Use alternative reagent to establish modified alkaline lysis method for extraction of plasmid DNA and use agarose electrophoresis to examine the quality of plasmid DNA, ultraviolet spectrophotometry to evaluate its production and purity, restricted enzyme cutting to examine its availability. [Results] The agarose electrophoresis showed that the plasmid DNA band was bright, mainly by form of superhelix. A260/280 was 1.903, DNA concentration was 320μg/ml, and we got 500 bp target fragment by restricted enzyme cutting. [Conclusion] We can get high purity of sample DNA by this simple and practical new method , it can meet the requirement of routine molecular biochemistry experiments.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

[Objective] Introduce a method for rapid extraction of Superior-Quality Plasmid DNA. [Method] Use alternative reagent to establish modified alkaline lysis method for extraction of plasmid DNA and use agarose electrophoresis to examine the quality of plasmid DNA, ultraviolet spectrophotometry to evaluate its production and purity, restricted enzyme cutting to examine its availability. [Results] The agarose electrophoresis showed that the plasmid DNA band was bright, mainly by form of superhelix. A260/280 was 1.903, DNA concentration was 320μg/ml, and we got 500 bp target fragment by restricted enzyme cutting. [Conclusion] We can get high purity of sample DNA by this simple and practical new method , it can meet the requirement of routine molecular biochemistry experiments.

Key concepts: Alkaline lysis, Plasmid preparation, Plasmid, Lysis, DNA, Agarose gel electrophoresis, Agarose, DNA extraction

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
A modified alkaline lysis method for extraction of plasmid DNA — Research Paper | ScholarLens