Detection of MRP of Streptococcus Suis Type 2 by PCR

A PCR assay for the rapid and sensitive detection of MRP of Streptococcus suis type 2 was developed.The PCR primers based on the MRP gene of S.suis type 2 could extend a 885bp PCR product.With Xba I digestion,the PCR product could produce a 578bp and a 307bp DNA fragment as expected.Using the same pairs no PCR product was detected from S.equisimilis,E.rhusiopathiae,P.pneumotropica, and M.hyopneumoniae.The PCR could detect 100 bacteria.Of 9 strains of S. suis type 2 isolated from diseased pigs,8 were positive.Of 15 porcine tonsillar specimens,1 was positive.The results demonstrated that the PCR is a highly specific and sensitive diagnostic tool for the detection of MRP from S.suis type 2 and can be used for fast diagnosis and epidemic survey.