Cloning,Expression and Analysis of Two Aquaporin1 Paralogous Genes in Cynoglossus semilaevis

We cloned the full length cDNA of two AQP1 from Cynoglossus semilaevis and sequenced it.Sequence analysis revealed that the fragment of AQP1o cDNA contained 789 bp open reading frame(ORF),120 bp 5′ untranslated region(UTR) and 84 bp 3′UTR and that of AQP1 cDNA contained 786 bp open reading frame(ORF),63 bp 5′ untranslated region(UTR) and 486 bp 3′UTR.The two sequences each encoded 262 amino acids and 261 amino acids,respectively.A phylogenetic tree was constructed with the sequence of AQP1o and AQP1 protein from different species.The AQP1o of Cynoglossus semilaevis was grouped together with some other marine fish's AQP1o mainly expressed in ovary.However the AQP1 of Cynoglossus semilaevis was grouped together with the AQP1 not expressed in ovary.This result showed that the two genes were different AQP1 homologous genes.RT-PCR analysis showed that the AQP1o was highly expressed in the ovary and slightly in the kidney of females,but not detected in the other tissues of females and any tissues of males.RT-PCR showed that AQP1 was expressed highly in some different tissues of females and males.This result indicated that the AQP1o might play an important role in ovary.