Cloning and Expression Analysis of DMRT 1 Gene in Cynoglossus semilaevis

The DMRT1 gene was obtained by homologous cloning in Cynoglossus semilaevis.The expression of the gene was analyzed in adult tissues and different developmental stages by RT-PCR.Sequence analysis revealed that the full-length cDNA was 1 232 bp containing 774 bp open reading frame(ORF), 131 bp 5′UTR,329bp 3′UTR.This sequence encoded 258 amino acids.Alignment analysis showed the amino acid sequence was 63%,59%,54%,53%,52%,38%,42%,41% identical to that from pejerrey,black sea bream,medaka,fugu,zebrafish,Africa clawed frogs,mouse and human,respectively.RT-PCR analysis showed that the gene expressed in all the analyzed developmental stages,from early embryo to 35-day juveniles,but the expression reached the highest level in juveniles at 22-days after hatching.In adult fish,however,its expression was detected only in male testis.This result indicated that the DMRT1 may play important role in the gonad differentiation and maturation of male C.semilaevis.