Protection of Exogenous Interleukin-10 on Gut Barrier in Acute Pancreatitis

Objective: To study the effect of exogenous IL-10 on gut barrier in severe acute pancreatitis(SAP).Methods: Ninety SD rats were randomly divided into three groups as the sham-operation group,SAP group and SAP with IL-10 treated group.Tweleve hours after operation,the indices in every group were recorded as following: the biochemical index,plasma endotoxin,organs bacterial translocation rate,ICAM-1 expression in gut mucosa,and the pathologic changes in pancreas and gut.Results: In SAP group,the plasma endotoxin was(144.682±9.584)EU/L,bacterial translocation rate was 64.6%,ICAM-1 expression(by flow cytometry) was 5.842±0.706,pancreas pathology score was 10.62±0.60,gut damage under microscope was severe.In IL-10 treated group,the above indices were significantly decreased as 103.112±8.124(P0.05),30.6%(P0.01),4.102±0.668(P0.05) and 4.46±0.52(P0.01),respectively,and the gut damage was ameliorated.Conclusion: The abnormal expression of ICAM in gut mucosa and the disorder of inflammatory interleukin both participated in the gut barrier disfunction during SAP.Early thearpy with exogenous IL-10 could alleviate ICAM-1 expression in gut mucosa,prevent inflammatory interleukin's releasing and decrease the bacterial translocation,which suggested a protective effect of IL-10 on gut barrier in SAP rats.