HLA-B~* 15 diversity in Guangdong Han population

Objective To investigate the allele distribution of HLA-B*15 gene family in GuangDong Han population.Methods A total of 466 samples with HLA-B*15 genotypes were selected from 1691 GuangDong Han individuals,based on their HLA-B types determined by PCR-SSP or PCR-SSO using commercial typing kits.PCR-SBT was applied to determine HLA-B*15 allele typing.Allele frequencies were analyzed by PyPop Software.And the chi-square values of allele frequencies from different populations were also analyzed.Results A total of 16 different HLA-B*15 alleles were identified.B*15∶02(64.75%) was the most frequent allele,followed by B*15∶01(13.26%),B*15∶25(4.554%),B*15∶27(4.158%),B*15∶12(3.960%),which comprised more than 90.69% of all the B*15-positive samples.In the B62 serologic group,seven alleles were identified,including B*-15∶01,B*1505,B*15∶25,B*15∶27,B*15∶35,B*1∶558.In the B75 serologic group,B*15∶02 and B*15∶11 were identified in the study samples and B*15∶02 was predominant.Further research is required to identify this unknown allele.Conclusion Our study demonstrates that the high diversity of B*15 in the GuangDong Han population,and provide that the basic data for the guidance of transfusion and drug hypersensitivity.