Influence of Jiang Tang Bu Shen Fang on expression of IKKβ mRNA in diabetic rats

Objective To observe the influence of Jiang Tang Bu Shen Fang on the expression of I kappa β kinase(IKKβ) mRNA in muscular tissue in diabetic rats induced by low-dose streptozotocin(STZ) and high-fat diet(HFD).Methods Male SD rats were fed HFD for 4 weeks,and then injected low-dose STZ.After one week the level of fasting plasma glucose was detected in rats and those with higher than 11.1 mmol/L of level of fasting plasma glucose were successfully modeled.The rats were randomly divided into the model group,low-dose,mid-dose and high-dose Jiang Tang Bu Shen Fang groups(low-dose,mid-dose and high-dose groups),aspirin group and blank group.All groups were intervened by relevant medicinal for 4 weeks.The expression of IKKβ mRNA in muscular tissue was detected by using reverse transcription polymerase chain reaction(RT-PCR).Results The expression of IKKβ mRNA in the model group was significantly higher than that in the blank group(5.098 times,P0.01).The expression of IKKβ mRNA in the aspirin group,high-dose and mid-dose groups was 2.814 times,3.246 times or 3.770 times,respectively lower than that in the blank group and showed P0.01 compared with that in the model group.In the low-dose group,the expression of IKKβ mRNA was 4.974 times lower than that in the blank group and showed P0.05 compared with that in the model group.Conclusion Jiang Tang Bu Shen Fang can inhibit IKKβ mRNA to some extent in diabetic rats,which may be one of mechanisms of the formula in the treatment of diabetes by inhibiting the conduction of inflammation signal pathway and improving insulin resistance.