2012•Zhongguo shiyan fangjixue zazhiRequires access
Controlling Effects of Neiyi Fang on Expression of mRNA and Protein of TGF-β_1 in Model Rats with EMS Iesions
Jiang Hui
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Abstract
Objective:To investigate the controlling effects of Neiyi Fang on expression of mRNA and protein of(TGF-β1) in endometriosis(EMS) lesions of model rats.Method:The model of EMS was duplicated in SD rats,rats were divided into sham group,model group,low-dose of Neiyi Fang group(25.2 g·kg-1·d-1),high-dose of Neiyi Fang group(50.4 g·kg-1·d-1) and western medicine(gestrinone) group(2.5 mg·kg-1·d-1).RT-PCR was used to detect the expression of mRNA of TGF-β1 in EMS lesions.Western blot was used to detect the expression of protein of TGF-β1.Result:The level of expression of mRNA and protein of TGF-β1 in the model group was clearly higher than that in the sham group(P0.01),while in the Neiyi Fang group,the level was clearly lower than that in the model group(P0.01).The level in the Neiyi Fang group was no significant difference than in the western medicine group.Conclusion:The expression of mRNA and protein of TGF-β1 in EMS lesions of model rats were increased.Neiyi Fang resisted EMS through decreasing the expression of mRNA and protein of TGF-β1.