Influence of acute high level glucose on rat bone formation

Objective To investigate the influence of acute high level glucose on rat bone formation in vivo and vitro.Methods Primary rat osteoblasts were incubated in medium containing control level glucose(5.5 mmol/L) and high level glucose(22.0 mmol/L) and were collected 24-,48-and 72-hour afterwards.Also,SD rats were infused with either glucose(50% glucose) or normal saline(control) for 24,48 and 72 hours and then sacrificed,and bone tissues of tibia and fibula were collected.Expressions of OC,COL1,RUNX2,ALP,c-JUN,IGF-1 mRNA and protein in primary osteoblasts and tissues of tibia and fibula were tested by real-time fluorescence PCR and Western blot.Results With the environment of acute high level glucose,expressions of ALP,COL1,RUNX2,IGF-1,OC,c-JUN mRNA were much higher in 48-hour treated group.Conclusions Proliferation and differentiation of osteoblasts in the process of bone formation may increase under the influence of acute high level glucose.The effect is modulated through MAPK signaling pathway.