RAPD Analysis of Genetic Diversity of Lycopersicum esculentum
Jingfu Li
Abstract
Jingfu Li
Abstract
Random amplified polymorphic DNA(RAPD)method was used to detect genetic variation of 63 varieties of tomato(Lycopersicum esculentum).From 180 amplified primer,and screened 22 of good stability,strong amplified polymorphic primers,RAPD primers generated 207 polymorphic bands.Percentage of polymorphic bands(PPB)of RAPD was 86.96%.The similarity coefficient between 0.28~0.99,By UPGMA cluster analysis,get and biology taxonomic position basic consistent results.
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Random amplified polymorphic DNA(RAPD)method was used to detect genetic variation of 63 varieties of tomato(Lycopersicum esculentum).From 180 amplified primer,and screened 22 of good stability,strong amplified polymorphic primers,RAPD primers generated 207 polymorphic bands.Percentage of polymorphic bands(PPB)of RAPD was 86.96%.The similarity coefficient between 0.28~0.99,By UPGMA cluster analysis,get and biology taxonomic position basic consistent results.
Key concepts: RAPD, UPGMA, Genetic diversity, Biology, Primer (cosmetics), Similarity (geometry), Botany, Genetic variation