Expression of Glutathione S-transferase-π in the Tissues of Ovarian Tumors and its Clinical Significance

ObjectiveTo study the expression of glutathione S-transferase-π(GST-π) gene in the tissues of ovarian tumors and its clinical significance. MethodsGST-π gene expression in tissues of 10 benign tumors and 40 malignant tumors was detected by immunohistochemical method of labeled streptavidin biotin(SP method).Chi-square test was used to analyze the interrelation between the expression of GST-π gene and histological type,pathological grade, clinical stage and preoperative chemotherapy. Results Positive expression of GST-π gene was detected in 26 cases out of 40 malignant ovarian tumors(65%); no positive expression was demonstrated in 10 benign ovarian tumors. There was no interrelation between expression of GST-π gene and histological types of tumor tissues and preoperative chemotherapy (P0.05). The positive expression rate in lowly differentiated cancer (85.71%)was higher than that in highly and moderately differentiated cancer (33.33%,P0.01), whereas positive expression rate of epithelial ovarian carcinoma in stage Ⅰ～Ⅱ and stage Ⅲ～Ⅳ were 20% and 71.4%,respectively. The expression rate might increase with the progress of tumor stage. The remission period of positive expression group was shorter than that of negative group, and the tumors were easily recurrent in recent days. ConclusionsDetection of GST-π gene expression rate shows certain clinical significance in the judgement of chemotherapy efficacy, overcoming of drug resistance and estimation of prognosis. Patients with positive GST-π gene expression are poor in chemotherapy efficacy and prognosis,for this positive expression belongs to drug resistance mediated by non-P-glycoprotein mechanism. [