The effect of Shenyanning on expression of extracellular signal-regulated protein kinase of human mesangial cells

Objective To investigate the effect of Shenyanning on the expression of extracellular signal-regulated protein kinase of human mesangial cells(HMCs).Methods HMCs were cultured by applying cell cultural technique.The serum pharmacological method was used in the preparation of shenyanning serum.HMC proliferation was detected by MTT.The p-ERK1/2 expression was detected by enzyme-linked immunosorbentassay(ELISA)taking lipopolysaccharide(LPS)as stimulating factor.Results Shenyanning significantly inhibited HMC proliferation;and the inhibition ratios of medium dose were 16.0%,15.4% and 18.2% when HMC were cultured for 12,24 and 48 h.The content of p-ERK1/2 in LPS group was obviously higher than that in blank group(P0.01),that in shenyanning group was obviously lower than that in blank group(P0.05).The expression of p-ERK1/2 in LPS combined Shenyanning group was obviously lower than that in LPS group(P0.01).Conclusions Shenyanning could inhibit the expression of p-ERK1/2 in the condition of normal or LPS stimulation,which may be the mechanism of Shenyanning in the delay of glomerularsclerosis.