Effect of "Yishenjuanshi" Mixture on Proliferation of Glomerular Mesangial Cell and the Phosphorylation of Extracellular Signal-Regulated Kinases

Objective To observe the effect of on the proliferation of glomerular mesangial cells(GMCs) and the phosphorylation of extracellular signal-regulated kinases(ERK).Methods All herbs in mixture were boiled in water and concentrated to different doses.After 7 days of treatment,serum from rats eye blood was collected.GMCs were cultured in serum free medium for 48hs and then 10% FSC-DMEM with or without serum including to detect GMCs proliferation and expression of phosphorylated ERK(p-ERK) by MTT and Western Blot respectively.Results Low,medium and high dose of Yishenjuanshi treatment significantly inhibited GMC proliferation when GMCs were cultured for 24,48 and 72 h;and the inhibition ratios increased as the dose increased.There was no difference in total ERK expression.However,Yishenjuanshi treatments with different dose all markedly inhibited p-ERK expression stimulated by FCS.Conclusion treatment inhibits GMC proliferation via blocking ERK phosphorylation.